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Zymopure ii plasmid miniprep kit

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50/740499. 240C-Miniprep—NucleoSpin Plasmid- Takara · D4200/D4201- ZymoPURE II Plasmid Midiprep Kit- Zymo  Ürün Kodu Ürün Adı Ambalajı Markası ZymoPURE D4200 ZymoPURE Midiprep Kit 25 Prep Zyppy™ Plasmid Miniprep Kit Zymoprep™ Yeast Plasmid Miniprep II. Tuotenumero PD1213-01, Kits määrä 50, Klikkaamalla linkkiä löydät tekniset tiedot tästä linkistä. 387 €. Prices subject to change without notice. 3. Plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (QIAGEN Inc. ZymoPURE ™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. coli overnight c ultures . 5-2. Zymo Research. 05 ml, 0. 6. Plasmid Miniprep Kit. Use these reagents for isolating and purifying nucleic acids from biological material for use in further laboratory procedures, and their ready-to-use Total price: $ 716. 3). The Norgen Plasmid Miniprep Kit is designed for the rapid purification of plasmid DNA from bacterial cultures. The kit includes sufficient reagents for 384 (4×96) minipreps. Plus shipping costs $45. 价格随时变动,展示价格仅供参考,下单前请咨询客服!. • Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl. coli DH5α harboring pCMVβ plasmid (7164 bp) was used for all comparisons. - Overnight bacterial culture with chassis containing plasmid of choice (1 – 5 mL) - Plasmid purification kit I. 50 ml ≤ 1 EU/µg 25 preps (D4200) 50 preps (D4201) ZymoPURE ™ Plasmid Maxiprep Kit 1200 µg 18 min. ¥ 1558. com: Zymo Research D4209 ZymoPURE Plasmid Miniprep Kit, 50 Preps: Industrial & Scientific ZymoPURE Plasmid Kits. x Convenient, ready-to-use plasmid purification kits and reagents are available for extracting from both bacterial and fungal cell types with high quality and yields. 96 preps. The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 ug of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Zymoclean Gel DNA Recovery. Estimated delivery period upon order confirmation (complete payment): ZymoPURE Plasmid Miniprep Kit, 100 Preps. 33 g of plastic waste (tubes, pipettes, pipette tips) was generated with traditional DNA purification, as compared to 11. The DNA isolated by this Kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-Time PCR and other downstream plasmid can be used for sequencing and other molecular cloning applications, including restriction enzyme digestion, transformation, PCR, in vitro translation and transfection. 129,60 ZymoPURE II Plasmid Midiprep fornece o método mais rápido e simples disponível para isolar com eficiência até 400 ug de DNA de plasmídeo de grau… ZymoPURE Plasmid MiniPrep Kit 50 Preps ≤ 15 minutes ≤ 5 ml ≥ 25 µl 100 µg ≤ 1 EU/µg D4210 100 Preps D4211 400 Preps D4212 800 Preps D4200 ZymoPURE™ II Plasmid Midiprep Kit 25 Preps ≤ 20 minutes 50 ml ≥ 100 µl 400 µg ≤ 0. Contents Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Quick View. 2mg of high-quality plasmid DNA from E. Rychlá a spolehlivá purifikace – až 100 μg plazmidové DNA díky spin-kolonkám; Inovativní ZymoPURE™ technologie umožňuje eluci ultra čisté plazmidové DNA bez endotoxinů dokonce i do pouhých 25 μl At the core of our ZymoPURE II technology is a novel binding chemistry and membrane that redefines plasmid purity, reduces processing time by 7 fold, and enables >1 mg of plasmid to be eluted from At the core of our ZymoPURE II technology is a novel binding chemistry and membrane that redefines plasmid purity, reduces processing time by 7 fold, and enables >1 mg of plasmid to be eluted from ZymoPURE Plasmid Miniprep Kit, 100 Preps. Available at Gentaur Genprice in 5 to 7 Working Days. NEB supplies all Monarch ® kit components separately. ZymoPURE Plasmid Miniprep Kit 质粒小提试剂盒,可分离转染 • Invitrogen ™ PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit • ZymoPURE ™ II Plasmid Maxiprep Kit* using the optional endotoxin removal step • Promega PureYield™ Plasmid Maxiprep System Bacterial strain and plasmid E. 25 ZD4201 ZymoPure Plasmid DNA Midi,50 preps 442. an understanding of the structure and function of plasmid DNAs. Catalog Code: D2004Product Unit: 50 preps. Simple method for yeast plasmid mini preparation. AxyPrep™ Plasmid Miniprep Kit by Axygen Scientific, Inc. List the function of each solution used in a plasmid DNA miniprep. Close. Specification. Will QuickClean II PCR Purification Kit remove fluorescent dyes from real-time PCR reactions? Are GenScript buffers with identical names in different kits the same? Can I use QuickClean Miniprep kits for low-copy plasmids and cosmids? How to resolve genomic DNA contamination in my plasmid prep? Stratagene’s QuikChange® II XL site-directed mutagenesis kit* is specifically optimized for large, difficult constructs and allows site-specific mutation in virtually any double-stranded plasmid. The unique ZymoPURE methodology removes ZymoPURE™ Plasmid Miniprep Kit Catalog Nos. 150 ml ≤ 1 EU/µg 10 preps (D4202) 20 preps (D4203) Bio Zyme este o companie specializată în importul și distribuția de kituri, reactivi, echipamente și consumabile de laborator, utilizate în special în universități, în centrele și institutele de cercetare, în spitale sau cabinete private, în industria farmaceutică precum și în toate instituțiile în care există laboratoare de biologie celulară și moleculară, genetică Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1. Therefore kits that can robustly isolate high quality, endotoxin-free plasmid DNA are indispensable tools in every researcher’s arsenal. Utilizing a modified Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Plasmid Miniprep Kit from Zymo Research Place a Zymo-Spin™ II-P Column in a Collection Tube and transfer the entire mixture. Using sterile technique, inoculate a single colony of E. With the vacuum off, add 800 µl of ZymoPURE™ Wash 1 to the Zymo-Spin™ II-P. Coupling ZymoPURE with the innovative The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. To confirm the insertion of GOI in the -cDNA vectorpLV, we recommend that the diagnostic digestion be performed with QuickClean II Plasmid Miniprep Kit provides a fast, simple, and cost-effective method for purification of up to 20 μg high-copy plasmid from 1–5 ml of overnight cultures of E. AxyPrep columns are designed for use in either a spin or vacuum format. 025 EU/µg D4201 50 Preps D4202 ZymoPURE™ II Plasmid Maxiprep Kit 10 Preps ≤ 20 minutes 150 ml ≥ 不,我们建议使用我们的ZR BAC DNA Miniprep试剂盒或ZymoPURE II试剂盒来分离大型构建体。 问题4:使用Zyppy试剂盒分离的质粒DNA中的内毒素水平是多少? ≤50内毒素单位/ µg质粒DNA。 Q5:我用完了Zyppy Wash Buffer。我可以用自制溶液或其他试剂盒中的洗涤缓冲液代替它吗? The Gene-Spin™ Plasmid MiniPreparation Kit is designed for the rapid, small-scale preparation of high-purity plasmid DNA. For 100-Cell Hinged Boxes. Zyppy. The recovered DNA is of a high quality, and is suitable for a number of downstream Plasmid DNA extraction and purification kit. ZymoPURE Plasmid miniprep (10 preps) – D4208S Fast and reliable purification of up to 100 µg of purified plasmid is predominately supercoiled and does not contain RNA or genomic DNA (Fig. Plasmid DNA MiniPrep 96-Well Kit. $240. Can the QuickClean II plasmid Miniprep Kit be used for isolating plasmid DNA from mammalian cells? No. Centrifuge 0. The ZymoPURE ™ Plasmid Midiprep kit features a spin column-based method for the purification of up to 300 µg of high-quality plasmid DNA in less than 18 minutes. The mini column has a plasmid DNA binding capacity of 50 µg. Plasmid DNA. and I had a very strange result because the plasmid on the gel looks like a digested one not as a circular plasmid. Miniprep. This kit uses the optimized alkaline lysis protocol and patented DNA binding matrix found in the Quantum Prep plasmid miniprep Kit. Typical yields from low copy number plasmids are highly dependent upon 400916 SideStep II Cell Lysis Analysis Kit 100 rxns 400902 SideStep miRNA Enrichment Kit 10 preps 400917 SideStep II qRT-PCR Master Mix, 1 Step 400 rxns DNA Purification 400761 StrataPrep Plasmid MiniPrep 50 rxns 400763 StrataPrep Plasmid MiniPrep Kit 250 rxns 720203 RecoverEase DNA Isolation Kit 15 preps 720202 RecoverEase DNA Isolation Kit 30 Order Plasmid Miniprep Kit (QuickClean II) (ABIN1536566) directly from us. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. On the same day I did the Mini for pGEM too, but this looks well. High copy-number plasmids typically yield 2–6 μg DNA/mL LB culture grown overnight. 商品描述:替换Qiagen12362. ZymoPURE-Express™ Plasmid Midiprep Kit. · Transfection-Grade: 50,000  ZymoPURE™ Plasmid Miniprep Kits, Zymo Research · Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column · Innovative  This is a little bit different, but I have used Zymo's miniprep kit and got poor DNA yield. Clean-up the product (usually I just use the Qiagen PCR purification kit) Convenient, ready-to-use plasmid purification kits and reagents are available for extracting from both bacterial and fungal cell types with high quality and yields. Discard supernatant. . SKU: D4019 分类: ZYMO 标签: 实验耗材. mosSCI plasmids were purified by ZymoPURE plasmid miniprep (Zymo #D4209). ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). Key to the kit is our proprietary DNA binding systems that allow the high efficient binding of DNA to our ezBind TM matrix while proteins and other contaminates are removed under certain optimal ZymoPURE ™ Plasmid Miniprep Kit 100 µg ≤ 15 min. Fastest: Simple 20 minute Maxipreps. The size of the bacterial culture used (and effectively the plasmid DNA yield) defines the plasmid preparation as miniprep, midiprep, maxiprep etc. The entire 20 minipreps procedure can be completed in 45 minutes or less, depending on the number of samples processed. Plasmid pQC -XIG (Addgene, catalog number: w497-1), deposited by Dr. 25 µg. Tuotenumero PD1213-02,Kits määrä 250, Klikkaamalla linkkiä löydät tekniset tiedot tästä Linkistä and uses either miniprep plasmid DNA or cesium-chloride-purified DNA. The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the ZymoPURE II Plasmid Isolation kits provide the fastest and simplest method  ZymoPURE Plasmid Miniprep Kit · Highest Yield: Purify up to 100 ?g of plasmid DNA in as little as 25 ?l directly from a spin-column. 2 mg) from E. D4213. Human embryonic kidney cells (HEK 293T, clone 17) (ATCC, catalog number: CRL-11268) 2. Storage Temperature: Room Temperature. Compare this item. Qiagen. Moreover, the isolated plasmid DNA is ultra-pure ZymoPURE II™ Plasmid Maxiprep (2 Preps) - D4202S; ZymoPURE™ Plasmid MiniPrep Kit (10preps) - D4208S; Zyppy Plasmid Miniprep Kit (10 Preps) - D4036S; Remarks ZymoPURE II™ Plasmid Maxiprep (2 Preps) - D4202S; ZymoPURE™ Plasmid MiniPrep Kit (10preps) - D4208S; Zyppy Plasmid Miniprep Kit (10 Preps) - D4036S; Remarks The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. TM. The systems allows to purify up to 40 ug plasmid from Plasmid Miniprep Kit II. 00 Zymoprep™ Yeast Plasmid Miniprep II (50 Preps) w/ Zymo-Spin™ I Columns. 250/740499. The Lab procudure can be found here. coli Inoculation 1. 93 ZD4203 ZymoPure Plasmid DNA Maxi,20 pre s 384. The Quantum Prep plasmid midiprep kit has been optimized for the rapid purification of DNA from 40 to 50 ml of liquid culture. page 8 Zymoprep™ Yeast Plasmid Miniprep Kits Simple solution for yeast plasmid The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E coli alkaline lysis method but using Zymolyase as the first solution The kit provides all the necessary reagents for plasmid isolation from S cerevisiae C albicans and S pombe and any fungi whose cell walls are susceptible to yeast Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. 55 / Each; Qty Add to cart Description; SDS Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. From these plasmid kits, the eluted plasmid DNA is ready for immediate use, without any alcohol precipitation. Rs. Catalog No. See page 3 for storage instructions. Promega. N. Prevent buffer retention and salt carry-over with optimized column design. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 20 minutes. 15 ZD4019 Zyppy Plasmid DNA, M in, 100 preps 105. ZymoPURE II™ Plasmid Gigaprep (5 preps) 417: D4208T: ZymoPURE Plasmid MiniPrep Kit (10 Preps) 418: D4209: ZymoPURE Plasmid MiniPrep Kit (50 Preps) 419: D4210: ZymoPURE Plasmid MiniPrep Kit (100 Preps) 420: D4211: ZymoPURE Plasmid MiniPrep Kit (400 Preps) 421: D4212: ZymoPURE Plasmid MiniPrep Kit (800 Preps) 422: D4213: ZymoPURE-Express ZymoPURE™ Plasmid Miniprep Kit & ZymoPURE™ II Plasmid Midi, Maxi, & Gigaprep Kits Quickly isolate large constructs (~200 kb) using a spin-column. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. b. 您的价格: ¥2880元. Name. ZymoPURE Plasmid Miniprep Kit, 50 preps, Fast & simple Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl. ZymoPrep Yeast Plasmid MiniPrep II Kit. Conclusions By using a modified protocol, twice as much low-copy number plasmid DNA can be purified in approximately 25 minutes with the ZymoPURE™ II Plasmid Maxiprep Kit. However, depending on the objectives of the experiment or the nature of the cloned insert, it is not always possible to use high-copy number plasmid DNA. Manufacturer: Zymo Research Corporation D4203. 其他信息. DNA Purif. ZyppyTM Plasmid Miniprep Kit ZymoPURETM Plasmid Midiprep Kit ZymoPURETM Plasmid Maxiprep Kit ZymoPURETM Plasmid Gigaprep Kit 2016. albicans and S. Total price: $ 716. The recovered plasmid can be used for Yeast transformations, Western blots, and PCR, etc. For 3-inch High Boxes. QuickClean II Plasmid Miniprep Kit provides a fast, simple, and cost-effective method for purification of up to 20 μg high-copy plasmid from 1–5 ml of overnight cultures of E. KIT CONTENTS The components included in the QuickClean 96-Well Plasmid Miniprep Kit are listed below. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. ZymoPURE II Plasmid Maxiprep Kit. Contain 100 columns and 100 collection tubes. ZymoPURE™ technology uses a modified alkaline lysis method and features novel ZD4200 ZymoPure Plasmid DNA Midi,25 preps 246. We offer a variety of suitable kits for your nucleic acid sample needs, including gDNA, RNA, and plasmid purification, single spin purification, nucleic acid amplification, nucleic acid gel extraction, PCR clean-up, and more. K4510-02 and K4500-02 refer to the manual supplied with the miniprep kit. Use a high copy number plasmid to obtain a good yield of plasmid DNA. 08 ZYMO ZY004 ZymoPure II Plasmid Midiprep Kit (25) $244. 1 This matrix, made up of the silicon dioxide exoskeleton of The objective of this lab is to perform extraction of plasmid DNA and analyze the results. page 2 - 4 Zyppy®-96 Plasmid Magbead Kit Pellet-free procedure for high-quality plasmid DNA (no centrifugation). sticky ends: Unpaired nuceotides at the end of a DNA Zymo PURE II plasmid kits . Request A Quote ZymoPURE II Plasmid Gigaprep Kit. Steel Dewar Flasks. ZymoPURE EndoZero Plasmid Maxiprep 无内毒性质粒大提试剂盒. coli, yielding 100–350 µg plasmid DNA from a bacterial culture with up to 50 preps. 产品中心. Digest plasmid following NEB protocol appropriate to the enzyme that you are using. E. (1) D4208t - Zymopure Plasmid Miniprep Kit - 10 Preps (1) D4209 This kit can provide 100 preps, recovering up to 200 µg of high copy plasmid DNA per prep. c ol i HB101. Reduce hands on time with faster protocols and less spin time. 5 ml ≤ 1 EU/µg 50 preps (D4209) 100 preps (D4210) ZymoPURE ™ Plasmid Midiprep Kit 300 µg 18 min. 15 USD ZymoPURE? Plasmid Miniprep Kit. ZymoPURE Plasmid MiniPrep Kit 50 Preps ≤ 15 minutes ≤ 5 ml ≥ 25 µl 100 µg ≤ 1 EU/µg D4210 100 Preps D4211 400 Preps D4212 800 Preps D4200 ZymoPURE™ II Plasmid Midiprep Kit 25 Preps ≤ 20 minutes 50 ml ≥ 100 µl 400 µg ≤ 0. Zymo-Spin™ II Columns (250 Pack) (Uncapped) ZymoPURE Syringe Plungers (5 pack) Zymoprep™ Yeast Plasmid Miniprep II (50 Preps) w/ Zymo-Spin™. The ZymoPURE - Express™ Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1. 12123 QIAGEN Plasmid Mini Kit (25) 20051 QIAEX II Gel Extraction Kit (500) 27104 QIAprep® Spin Miniprep Kit (50) Purification of up to 1. OBJECTIVES After completion, the student should be able to: 1. For long (~8 kb) or difficult targets, we offer the QuikChange II XL Site Directed Mutagenesis Kits (Catalog #200521 and #200522). QIAprep Spin Miniprep Kit (50) (QIAGEN, catalog number: 27104) Cells and Plasmids . DNA extraction Whole DNA (includes both chromosomal and plasmid DNA) from the five S. Eric Campeau BioVision’s Plasmid Miniprep kit is designed for fast and efficient purification of plasmid DNA from 1-4 mL of E. 5-5 ml of bacterial culture in a clear 1. The yield and quality of miniprep plasmid DNA purified by using Favorgen plasmid DNA extraction mini kit (Catalog # FAPDE 001) is sufficient for generating 30 ml of lentivirus. Perform a plasmid DNA miniprep. ZymoPURE™ Plasmid Miniprep: 15 minute prep with a unique chemistry to ZymoPURE™ II Plasmid Midiprep and Maxiprep Zymoclean™ Gel DNA Recovery Kit. 100 tests. A. 00 ZD4020 Zyppy Plasmid DNA, M in, 400 preps 369. This reagent does not need to be removed before RNA extraction with the Monarch Total RNA Miniprep Kit. This kit provides a rapid and economical method for the purification of plasmid DNA in a convenient mini column format. FREE PREMIUM IPTG, 5G $ 66. ZymoPURE Plasmid Miniprep Kit 质粒小提试剂盒,可分离转染级质粒 . no. The wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. SKU: D4209 Categoria: Zymo Research Tags: plasm?deo, Plasmid, quiagen, zyppy. Add the proper amount of antibiotics to the LB broth. coli cultures in less than 20 min. Tube Storage Boxes w/ Lids. ® Plasmid DNA Mini Kit I. 04 g (Table 2)—a 75% difference in waste generation. and uses either miniprep plasmid DNA or cesium-chloride-purified DNA. DNA Recovery. Place your Order Online or by Email to info@gentaur. coli culture in less than 20 min. Avaliações (0) Avaliações Não há avaliações ainda. For kit components of the PureLink ® Quick Plasmid Miniprep Kit (Box 3) supplied with Cat. These plasmid kits feature ZymoPURE II technology, including ZymoPURE II Plasmid Maxiprep (2 preps) – D4202S ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA (up to 1. a. ZYMO RESEARCH. x. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midipr Fastest and simplest method to isolate endotoxin-free plasmid DNA. ZymoPURE Plasmid MiniPrep Kit ZYR334208 ZYR D4208T 10 Preps 1 kit / 6,000 ZYR034209 ZYR D4209 50 Preps 1 kit / 16,000 ZYR534210 ZYR D4210 100 Preps 1 kit / 30,000 ZYR034211 ZYR D4211 400 Preps 1 kit /109,000 ZYR034212 ZYR D4212 800 Preps 1 kit /193,000 Miniprep以外のサイズもあります。 その他のZymoPUREシリーズ Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. 库存 9 件. 2012), which was grown on E . ZymoPURE II Plasmid Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. FIGURE 1 Overview of the QuikChange II site-directed mutagenesis method. The ZymoPURE™ Plasmid Maxiprep kit features a spin column-based method for the purification of The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The ZymoPURE™ II Plasmid Midiprep Kit features a simple spin-column based method for the purification of up to 400 µg of transfection grade plasmid DNA in less than 20 minutes. 150 ml ≤ 1 EU/µg 10 preps (D4202) 20 preps (D4203) The ZymoPURE™ Plasmid Gigaprep kit features a spin column-based method for the purification of up to 10 mg of high-quality plasmid DNA in less than 50 minutes. 60 ZYMO ZY003 ZymoPure II Plasmid Maxiprep Kit (20) $382. ZymoPURE Plasmid MiniPrep Kitまたは他社製品を用いて抽出・精製したプラスミドDNAの収量・濃度の比較. ZYMO Zyppy Plasmid Miniprep Kit Zyppy质粒微量制备试剂盒 quantity. The Kit provides a fast, simple, and cost-effective plasmid miniprep method for 1–5 ml of overnight cultures of E. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. A4001-25. . D4200. 商品规格: 10T. K4575-02) is shipped with an additional box containing reagents for plasmid purification (Box 3). 00. Continued on next page ChargeSwitch®-Pro Plasmid Miniprep Kit with traditional technology showed that 44. (1) D4203 - Zymopure Ii Plasmid Maxiprep Kit - 20 Preps. Z. Use the designated filters to select for the purification scale (from miniprep to gigaprep), format (spin, vacuum, or magnetic), and compatibility with automated systems. Co-injected plasmids were purified by HiSpeed Plasmid Midi kit (Qiagen #12643). Plasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications. 80 ZYMO ZY002 ZymoPure Plasmid MiniPrep Kit (100) $148. com. EZ RNA Methylation Kit. Application: DNA Purification. For plasmid isolation from S. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1. It is a solution for the preservation of nucleic acids in various tissue types. 2 mg of high-quality plasmid DNA in less than 18 minutes. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry, which enables the highest yields and concentration of plasmid DNA (up to 3 µg/µl) directly from a spin-column. 80 ZYMO 3/26/21 Page - 1. 5ml overnight cultures. The ZymoPURE ™ Plasmid Maxiprep kit features a spin column-based method for the purification of up to 1. coli containing your plasmid from an agar plate into LB broth. Plasmid Miniprep Kit . 01r 0. The eluted plasmid DNA is EndoZero and ready for immediate use in the most sensitive applications. ZymoPURE Plasmid Miniprep video The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Quick-DNA Fungal/Bacterial Miniprep Kit. ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. Plasmid Extraction from Bacteria (miniprep) Purification o f p lasmid D NA from 1 – 5 m l E . This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E. (ZymoPURE Plasmid Miniprep Kit) Watch the Youtube video for questions about the Miniprep protocol. Coli. Fastest and simplest method to isolate endotoxin-free plasmid DNA. 00 ZD4201 ZymoPure Plasmid DNA Midi,50 preps 458. 5 In addition, the QuikChange II I used by the first time the GenElute HP Plasmid Miniprep kit to do the MiniPrep of E- Coli transformed with PCR Blunt II-TOPO Vector. Description Specifications. Important Notes The yield of plasmid DNA using the Miniprep-II kit depends on: 1) The origin of the replication. 1. 35 ZD4204 Zy mo Pure las idDNAGiga,5 pre s 446. 00 ZD4204 ZymoPure Plasmid DNA Giga ,5 preps 462. Are manufactured using less plastic than other leading suppliers. The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of up to 20 μg of high quality plasmid DNA. Yields of up to 25 µg super coiled plasmid DNA per preparation. Bacterial culture 首页. 31 TM ZymoPURE EZ-Vac TM Vacuum Manifold Cat. The ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. coli from an overnight LB culture (0. 00 Excl. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. 50-197-7585. Zyppy Plasmid Miniprep Kit. The PureYield™ Plasmid Miniprep Start-Up Kit contains 750 minipreps, a vacuum manifold and a free vacuum pump. ZymoPUR Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process • Invitrogen ™ PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit • ZymoPURE ™ II Plasmid Maxiprep Kit* using the optional endotoxin removal step • Promega PureYield™ Plasmid Maxiprep System Bacterial strain and plasmid E. 50 applications. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of We typically combined >0. I am using the Zymopure plasmid miniprep kit and I have repeated my extractions at least 6-7 times now. ZymoPURE technology uses a The ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1. 1 mL, 1 mL, 3 mL and 5 mL culture, lanes 1- 5 respectively), using ISOLATE II Plasmid Mini Kit and run on 1. ZymoPURE technology uses a modified alkaline lysis method and features novel The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. ZymoPURE. The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. 3ng for most 2µ r based plasmids from 1. Use these reagents for isolating and purifying nucleic acids from biological material for use in further laboratory procedures, and their ready-to-use Zymoprep Yeast Plasmid Miniprep II: D2004 : Zymo Research Corp. Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. DETAILS. restriction enzyme: Enzyme that cuts "cleaves" DNA at a specific sequence of bases. ZymoPURETM II Plasmid Midiprep Kit (Genesee Scientific, catalog number: 11-550B) 25. Monarch. Item. coli cells containing your plasmid in LB broth. 19 oct. ZymoPURE Plasmid Maxiprep Kit 质粒大量提取试剂盒. 2 mg of transfection grade plasmid DNA from E. Plasmids differ significantly in copy number due to their origin of replication, size, and associated insert. coli. 用户评论 (0) For fast and efficient purification of plasmid DNA from 1-15 mL of E. Store Box 1 at -20°C, Box 2 at -80°C, and Box 3 at room temperature. Features & Benefits: For fast and efficient purification of plasmid DNA from 1-15 mL of E. For fast and efficient purification of plasmid DNA from 1-15 mL of E. Mini-prep Isolation of . The PureLink® HiPure Plasmid Filter Purification kits allow you to purify all types and sizes of plasmid DNA. coli in less than 18 minutes. 34 Plasmid Miniprep Kit. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of ZymoPURE II Plasmid Maxiprep (2 preps) – D4202S ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA (up to 1. 25900-NB. With the binding capacity of 80 µg, the yield obtained by Miniprep Kit II (K1313) is higher than the Miniprep Kit I 24. Add to wishlist. 50 rxns. The kit eliminates the need for subcloning and for ssDNA rescue. ZymoPURE II Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. U p d a t e d R e v i s e d a n d Order Plasmid Miniprep Kit II (ABIN3045596) directly from us. The unique spin-column design also provides zero buffer retention and a low elution volume. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps. ZD4200 ZymoPure PlasmidDNA Midi,25 preps 255. D4203/D4202- ZymoPURE II Plasmid Maxiprep Kit- Zymo Research » EAD SCIENTIFIC. Plasmid Miniprep Kit; Plasmid Midiprep Kit; Plasmid Maxiprep Kit; Plasmid Megaprep; 96-well Plasmid Miniprep; Magnetic Beads Based Isolation; Endofree Plasmid Isolation; EndoFree ezFlow Plasmid Isolation; endA+ Strains; High-Capacity Vectors; Low Copy Number Plasmid Purification Kit; Yeast Plasmid Isolation; Uracil template preparation kit broth for miniprep Day after growing Perform miniprep plasmid 1 h bacterial culture for purification to isolate miniprep plasmid carrying insert Next laboratory activity Digest plasmid DNA with 1 h Bgl II restriction enzyme Analyze digest by agarose gel 1 h electrophoresis Prepare the DNA insert for 0. 55 / Each; Qty Add to cart Description; SDS The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, and DNA sequencing. Students will develop . 3. NEB. Plasmid. The PureLink HiPure Plasmid Midiprep Kit is used to isolate transfection-grade plasmid DNA from E. 描述. Elute in as little as 30 μl. GST. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. Supplier: MyBiosource. $306. ZymoPURE II™ Plasmid Maxiprep (20 preps) 20次: D4204: ZymoPURE II™ Plasmid Gigaprep (5 preps) 5次: D4208T: ZymoPURE Plasmid MiniPrep Kit (10 Preps) 10次: D4209: ZymoPURE Plasmid MniPrep Kit (50 Preps) 50次: D4210: ZymoPURE Plasmid MniPrep Kit (100 Preps) 100次: D4211: ZymoPURE Plasmid MniPrep Kit (400 Preps) 400次: D4212: ZymoPURE ZYMO Zyppy Plasmid Miniprep Kit Zyppy质粒微量制备试剂盒. Request A Quote Zymoprep Yeast Plasmid Miniprep II. If using the QIAprep® miniprep kit from QIAGEN: 1. Maybe 9 ng/uL DNA, whereas I can sometimes get >1 ug/uL DNA  ZymoPURE. EZ-96 DNA Methylation Kit. 5,351. Monarch RNA Purification Columns: Are a component of the Monarch Total RNA Miniprep Kit ( NEB #T2010 ) Have a binding capacity of up to 100 µg of total RNA. The kit allows for the removal of RNA, SDS, genomic DNA and other contaminants. Each AxyPrep column has a binding capacity of at least 20 mg of plasmid DNA. 5. 2020 DNA was then extracted with ZymoPURE™ Plasmid Miniprep Kit (Zymo Research). FEATURES. Plasmid Miniprep (cat. (1) D4202 - Zymopure Ii Plasmid Maxiprep Kit - 10 Preps. 025 EU/µg D4201 50 Preps D4202 ZymoPURE™ II Plasmid Maxiprep Kit 10 Preps ≤ 20 minutes 150 ml ≥ Gel extraction kit (EZ, catalog number: M1002-50 ) One Shot TM TOP10 Chemically Competent E. 商品列表. coli in less than 18 minutes. Kit Components • ezBind Columns • Buffer A1 • Buffer B1 • Buffer N1 • Buffer KB • DNA Wash Buffer Oligo Clean & Concen. Grow E. Macherey-Nagel. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of Amazon. Add to cart. ZymoPURE Syringe Filters and Punger Set (5 pack). Description. Buffer A1, B1, N1, KB, DNA Washing Buffer, Elution Buffer, RNase A, ezBind Columns(50), User Manual(1) Introduction. View more product information. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midipr The ZymoPURE™ II Plasmid Midiprep Kit features a simple spin-column based method for the purification of up to 400 µg of transfection grade plasmid DNA in less than 20 minutes. 0 and the 260/230 Miniprep was performed with ZymoPURE Plasmid Miniprep Kit from Zymo Research. K1445-100-BV. 产品简述: 其他名称: 质粒小提 商品品牌:Zymo Research CAS号: 库房: ZymoPURE-EndoZero™ Plasmid Kits. El kit ZymoPURE ™ Plasmid Miniprep presenta un método basado en una columna giratoria para la purificación de hasta 100 µg de ADN plasmídico libre de endotoxinas ultra puro en menos de 15 minutos. BioVision’s Plasmid Miniprep kit II is designed for fast and efficient purification of plasmid DNA from 1-15 mL of E. Monarch ® Plasmid Wash Buffer 2. Zyppy Plasmid Miniprep Protocol A. The total plasmid yield is 0. Manufacturer: Zymo Research Corporation D4209. For 15 & 50ml Tube Boxes. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights • Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column. The table below will help you choose the right plasmid DNA midiprep kit. Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: orders@genomics-online. Application support available for new users & trial arrangements. Suitable for the simultaneous processing of multiple samples. ZymoPURE™ Plasmid Miniprep Kit ZymoPURE - Express™ Plasmid Midiprep Kit Plasmid Miniprep Kit. 2. For 2-inch High Boxes. Zymoclean Gel . Vocabulary: plasmid: Small, typically circular, strand of DNA. ZymoPURE ™ Plasmid Miniprep Kit 100 µg ≤ 15 min. D4208T. ABIN3045596. 4 jun. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Plasmid Wash Buffer 2. 2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without pelleting and resuspension steps. ZymoPURE Plasmid miniprep (10 preps) – D4208S Fast and reliable purification of up to 100 µg of For 15 & 50ml Tube Boxes. 3/17/2017: ZymoPURE II Plasmid Midiprep Kit, 25 preps & 50 preps: D4200 & D4201 : Zymo Research Corp. Bacterial culture The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. 00 ZD4202 ZymoPure Plasmid DNA Maxi,10 preps 206. 10 preps. $187. 29 ZD4202 ZymoPure Plasmid DNA Maxi,10 preps 198. A3001-15. While the yield is okay (around 350ng), the 260/280 ratio is around 1. Add to Cart. Plasmid Miniprep Purification Kit Features - Spin column format and easy to use - High yield and high purity - Speedy operation procedure Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. ZymoPURE Plasmid Miniprep Kit, 100 Preps. The DNA isolated by this Kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-Time PCR and other downstream pUC19 plasmid was isolated from increasing volumes of E. coli (up to 400 µg in 18 minutes). 12123 qiagen plasmid mini kit (25) 12163 qiagen plasmid maxi kit (25) 12262 qiafilter plasmid maxi kit (10) 12643 hispeed plasmid midi kit (25) 12662 hispeed plasmid maxi kit (10) 20021 qiaex ii gel extraction kit (150) 201203 taq dna polymerase (250 u) 203603 hotstartaq plus dna polymerase (250) 203605 hotstartaq plus dna polymerase (1000) Edvo-Kit #202. Experiment Objective: The objective of this experiment is to introduce the principles of extracting plasmid DNA from bacterial cells. coli (Invitrogen, catalog number: C404003 ) ZymoPURE TM II Plasmid Midiprep Kit (Genesee Scientific, catalog number: 11-550B ) QIAprep Spin Miniprep Kit (50) (QIAGEN, catalog number: 27104 ) Cells and Plasmids 11-315 | Yeast Plasmid Miniprep II,Zymo Research Kit size: 50 Preps/Unit | 259. (1) D4201 - Zymopure Ii Plasmid Midiprep Kit - 50 Preps. Types of Kits TOPO TA Cloning® Kits for Sequencing are available with either Mach1™-T1R, The purity, quality, and quantity of your nucleic acid samples depends on reliable nucleic acid purification reagents and kits. Dále také kit v mini verzi z edice ZymoPURE: D4208S: Free sample: ZymoPURE™ Plasmid Miniprep . ZymoPURE technology uses a The ZymoPURE Plasmid Gig prep kit features a spin column-based method for the purification of up to 10 mg of high-quality plasmid DNA in less than 50 minutes. Verify the success of a plasmid DNA miniprep using gel electrophoresis. 55 / Each; Qty Add to cart Description; SDS ZymoPURE™ II Plasmid Maxiprep Kit, 20 Preps. 50/740588. 00 each – + Add to cart Plasmid Miniprep Kit II, Kits määrä 50 tai 250. Add 250 μl of ZymoPURE P1 (Red) to the bacterial cell pellet and resuspend completely by vortexing or pipetting. 5% TAE agarose gel. 00 ZymoPURE™ Plasmid Miniprep Kit, 50 preps. The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. It is supplied as a 2X concentrate. Cat# MBS846529. Product Contents: ZymoPURE™ Plasmid Miniprep. For unc -119(+ )selection, plasmids were injected into strain EG6699 (Frokjaer-Jensen e t al . 26 ZD4020 ZyppyPlasmidDNA,M in, 400 preps 356. Fiberboard Boxes (-80°C) ZymoPURE ™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli' cells (see: Protocol. ChargeSwitch® Kits Comparison to traditional purification methods Green Benefits Monarch ® DNA/RNA Protection Reagent is a component of the Monarch Total RNA Miniprep Kit. For our first lab we created DNA ladders using pPSU1 and pPSU2 using the ZymoPure Plasmid Miniprep Kit. nos. Miniprep Scale ZymoPURE™ Plasmid Miniprep Kit Rapid purifi cation of ≤ 100 μg transfection-grade plasmid DNA Midiprep, Maxiprep, and Gigaprep Scales ZymoPURE ™ Kits ZymoPURE-EndoZero ZymoPURE-Express Transfection-ready plasmid DNA in ≤18 minutes, using a spin-column: Midi ≤ 300 μg Maxi ≤ 1200 μg Giga ≤ 10 mg Ultra-low ZymoPURE™ II Plasmid Midiprep Kit, 25 preps. PureYield™ Minipreps rapidly isolate up to  使用例. Supplied as a 5X concentrate; add ethanol before use. 您的价格: ¥2356元. Valencia, CA). ZymoPURE Plasmid Miniprep video Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. (Uncapped). For long (~8 kb) or difficult targets, Stratagene offers the QuikChange II XL site directed mutagenesis kits (Catalog #200521 and #200522). Manufacturer: Zymo Research Corporation D4200. ZymoPURE™ technology uses a modified alkaline lysis method and features The yield of the high-copy plasmid from this kit is about 30-50µg. This item: ZymoPURE Plasmid Miniprep Kit, 400 Preps $ 560. ZY005 Zymoclean Gel DNA Rec Kit (50) w/columns $81. D4036. Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Learn more about Monarch Nucleic Acid Purification Kits. This kit also works well with low copy number yeast plasmids. 00 ZD4203 ZymoPure 2Plasmid DNA Maxi, 0 pre s 398. 5 million colonies and purified plasmid with a ZymoPURE II Plasmid Maxiprep Kits (Zymo Research) to make the full-length recombinant hyperimmune globulin maxiprep library QuickClean II Plasmid Miniprep Kit provides a fast, simple, and cost-effective method for purification of up to 20 μg high-copy plasmid from 1–5 ml of overnight cultures of E. FIGURE 1 Overview of the QuikChange II-E site-directed mutagenesis method. 96-Well Plate. II. Plasmid Miniprep Kit II. 00 ZD4019 Zyppy Plasmid DNA, M in, 100 preps 109. Kit. ZymoPURE IIエンドトキシン濃度. 2016 The yield was lower than I anticipated (I got 100 ug per plasmid, I expected ~250 ug/plasmid based on preliminary minipreps, Zymo says 300 ug is  Plasmid DNA Isolation. #S7000 ZymoPureTM EZ-Vac TM Vacuum Manifold ZyppyTM 7 ZYMO RESEARCH The Beauty of Science is to Make Things Simple $ 9500 $4999 ZymoPURE II Plasmid Midiprep Kit, 50 Preps R$ 5. Fiberboard Boxes (-80°C) The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Anti-5-Methylcytosine Monoclonal Antibody (Clone 10G4) 15 µg. cerevisiae, C. flexneri isolates was extracted using the High Pure PCR Template Preparation Kit (Roche Molecular Biochemicals, Indianapolis, IN). miniprep. 5 h sequencing DNA Extraction: Article Snippet: . (1) D4204 - Zymopure Ii Plasmid Gigaprep Kit - 5 Preps. Several plasmids contain mutations that allow them to reach very high-copy numbers within the bacterial cell. The unique ZymoPURE methodology removes The ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. Plasmid DNAs purified from this kit can be used directly for downstream applications such as transfection, cloning, sequencing, as well as in vivo applications. Nowadays, a variety of convenient and fast plasmid DNA purification kits are commercially available ensuring advanced quality and purity (see Explanatory Chapter: How Plasmid Preparation Kits Work). A component of the Monarch Plasmid Miniprep Kit ( NEB #T1010) Used as the second wash buffer in the miniprep workflow to remove residual buffer components. Uses a modified alkaline lysis method. The kit can also be used to prepare plasmid DNA in a concentrated form. Rnase A, 30 mg $ 90. 5 ml tube at full speed for 15- 20 seconds in a microcentrifuge. 4. Anti-5-Hydroxymethylcytosine Polyclonal Antibody. ZR Plasmid Miniprep™ – Classic. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture  The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less  Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg  The Quick-DNA™ Miniprep Plus Kit yields highly ZymoPURE™ II Plasmid Midiprep Kit Stated manufacturers' endotoxin levels for the ZymoPURE™ II. The DNA isolated by this Kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-Time PCR and other downstream Plasmid Miniprep Kit II Kit. Plasmid DNA samples were used as a template for the generation  PLASMID KITS · 740588. ZR BAC DNA Miniprep Kit. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, and DNA sequencing. The results illustrate the very high binding capacity of the columns in the ISOLATE II Plasmid Mini Kit. Kit Size. com Plasmid Miniprep Kit II (up to 80ug), 250 Preps PD1213-02 $280. ZymoPURE II Plasmid kits are the fastest and simplest plasmid kits available to efficiently isolate transfection-grade plasmid DNA from E. 专栏中心 ZymoPure Plasmid Maxiprep Kit 10 preps D4202: each: Zymo Research: 121257: Zyppy Plasmid Miniprep 50 preps D4036: each: Zymo Research: 57665: DNeasy Blood & Tissue D4200 - Zymopure Ii Plasmid Midiprep Kit - 25 Preps. What is the recommended culture medium for the Plasmid miniprep kit? LB culture medium Zymoprep Yeast Plasmid Miniprep Kit II (Zymo Research) Reagents for transformation of competent 'e. The ZymoPURE Plasmid Midiprep kit features a spin column-based method for the purification of up to 400 µg of high-quality plasmid DNA in less than 18 minutes (). ZymoPURE Plasmid Miniprep Kit. For 96-Well Plates. D4205. D4015.